|Activity of G6PD in Stored Blood Orient Journal of Medicine Vol 26 [3-4] July-Dec, 2014|
The activity of glucose-6-phosphate dehydrogenase (G6PD) in stored blood
1Silas A UFELLE1
2Emeka E NEBOH2
4Ebele J IKEKPEAZU
5Ignatius C MADUKA
1Dept of Medical Laboratory Sciences
3Dept of Haematology & Immunology
4Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu, NIGERIA
2Department of Medical Biochemistry, College of Medicine Enugu State University of Science & Technology Enugu, NIGERIA
5Department of Chemical Pathology, University of Nigeria Teaching Hospital
Author for Correspondence
Emeka E NEBOH
Department of Medical Biochemistry, College of Medicine, Enugu State University of Science & Technology Enugu NIGERIA
Received: May 6th, 2014
Accepted: September14th, 2014
Background: Blood transfusion is a critical component of supportive therapy. Red blood cell viability in stored blood determines successful transfusion. Glucose-6-phosphate dehydrogenase (G6PD) activity has been shown to maintain red blood cell membrane integrity. This study was, therefore, aimed at estimating the G6PD activity in stored blood bags at the blood bank of the University of Nigeria Teaching hospital (UNTH) Enugu.
Methodology: The activity of G6PD in 100 stored blood bags consisting of different ABO groups [A (n=30); B (n=30); O (n=30) and AB (n=10)], stored at the blood bank of the UNTH Enugu between April and August 2009, was determined using methhaemoglobin reduction and ultraviolet (UV) spectrophotometric quantitative methods. The data obtained were statistically analyzed using studentís t-test and analysis of variance.
Results: There was statistically significant decrease in the G6PD activity from the third week of storage (p<0.05) at the blood bank, under optimum storage conditions. The different ABO blood groups did not show any significant variation (p>0.05) in G6PD activity.
Conclusion: Storage of whole blood for up to three weeks results in significant decrease in the G6PD activity and possibly, affects the red cell viability. Stored blood in the blood bank should be used up before the third week to ensure viability of red blood cells.
Keywords: ABO groups, blood transfusion, blood donors, haemolytic anaemia, red blood cells